The Hanging Drop Slide Technique to Study Bacterial Motility
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چکیده
In this exercise you will learn to make hanging drop slides and then use this technique to observe the motility of living bacteria. Motility is an important characteristic used to identify microorganisms. Three bacteria will be used which vary in size, shape and arrangement of flagella, and types of motion. Specifically you will observe Pseudomonas aeruginosa, a monotrichous bacterium that exhibits high motility using a polar flagellum, Bacillus cereus that moves by peritrichous flagella and Spirillium volutans, a helical cell that moves using large bipolar tufts of flagella (possible mixture of amphitrichous and lophotrichous organisms) (Refer to Figure 1).
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